The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. To detect mJE by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mJE.
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of mJE (100 ng/ml), a concentration of 4.0 - 6.0 µg/ml of this antibody is required.
Use at an assay dependent concentration. Can be blocked with Recombinant mouse MCP1 protein (ab9901). To detect mJE by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 1 µg/ml.
FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
Post-translational modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
staining in colchicine injected mouse brain (including caudate putamen) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.
References for Anti-MCP1 antibody (ab9899)
has not yet been referenced specifically in any publications.
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