• Product nameAnti-MCP1 antibody [ECE.2]
    See all MCP1 primary antibodies
  • Description
    Rat monoclonal [ECE.2] to MCP1
  • SpecificityThe antibody reacts with murine JE / MCP-1.
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 102-130 of Mouse MCP1

  • General notesMonocyte chemotactic protein-1 (MCP-1) is also known as MCAF.



Our Abpromise guarantee covers the use of ab8101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150. PubMed: 15829914
IHC-Fr Use at an assay dependent dilution. PubMed: 19920349
IHC-P 1/50.
WB 1/50.


  • FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • GDCF 2 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody [ECE.2] images

  • Immunohistochemical analysis of formalin-fixed murine duodenal tissue, staining MCP1 with ab8101.

References for Anti-MCP1 antibody [ECE.2] (ab8101)

This product has been referenced in:
  • Lau KS  et al. Multi-Scale In Vivo Systems Analysis Reveals the Influence of Immune Cells on TNF-a-Induced Apoptosis in the Intestinal Epithelium. PLoS Biol 10:e1001393 (2012). IHC-P ; Mouse . Read more (PubMed: 23055830) »
  • Zhang H  et al. Interferon-gamma induced adipose tissue inflammation is linked to endothelial dysfunction in type 2 diabetic mice. Basic Res Cardiol 106:1135-45 (2011). Read more (PubMed: 21826531) »

See all 6 Publications for this product

Product Wall

Thank you for your reply.

I can confirm this antibody ab8101 is not conjugated. If an antibody is conjugated, this information is provided on the datasheet.

I can recommend to consider conjugating the antibody with a conjugate of ...

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Thank you for contacting us. Yes, this antibody has been tested and guaranteed for use in IHC. Please see the references for images.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or...

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Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

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Thank you for your enquiry. For a positive tissue control for ab8101, we recommend any vascular tissue, for instance spleen or lung. For antigen retrieval, we do not have information on which AR treatment, if any, is optimal for this antibody. We...

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Thank you for your enquiry. We're currently aware of one publication which featured the use of this antibody: Zoja C et al. Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. J Am Soc Nephrol 8:720-9 (1997). PubMed: 91...

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Thank you for your question regarding ab8101. The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

The molecular weight of predicted mouse MCP-1 is 12 kDa. The expressed protein has a molecular weight of 12-25 kDa. Variable O-linked glycosylation gives rise to the variation in molecular weight.

The antibody reacts with murine JE / MCP-1, but it has not yet been tested for use with paraffin embedded tissue.