Anti-MCP1 antibody [S101] (ab18678)

Overview

  • Product nameAnti-MCP1 antibody [S101]
    See all MCP1 primary antibodies
  • Description
    Mouse monoclonal [S101] to MCP1
  • Specificityab18678 reacts with natural and recombinant human MCP1. It does not react with human interleukin 8 (IL8) and other human cytokines tested such as interleukin 1 beta (IL1 beta), serum amyloid A (SAA) and epidermal growth factor (EGF).
  • Tested applicationsSuitable for: ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab18678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Neutralising
WB
  • Application notesELISA: Use at an assay dependent dilution.
    Neut: Use at a concentration of 0.5 µg/ml.
    WB: Use at a concentration of 0.1 - 5 µg/ml. At a concentration of 0.1 - 1.0 µg/mL the antibody will allow visualization of 0.1 µg/lane of human MCP1. Concentration of 5.0 µg/mL will allow visualization of 50 µg/lane of rabbit MCP1. Predicted molecular weight: 11 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
    • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications
      Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-C motif chemokine 2 antibody
      • CCL2 antibody
      • CCL2_HUMAN antibody
      • Chemokine (C C motif) ligand 2 antibody
      • GDCF 2 antibody
      • GDCF-2 antibody
      • GDCF2 antibody
      • HC11 antibody
      • HSMCR30 antibody
      • JE antibody
      • MCAF antibody
      • MCP 1 antibody
      • MCP-1 antibody
      • MCP1 antibody
      • MGC9434 antibody
      • Monocyte chemoattractant protein 1 antibody
      • Monocyte chemotactic and activating factor antibody
      • Monocyte chemotactic protein 1 antibody
      • Monocyte secretory protein JE antibody
      • SCYA2 antibody
      • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
      • Small inducible cytokine A2 antibody
      • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
      • Small-inducible cytokine A2 antibody
      • SMC CF antibody
      • SMC-CF antibody
      • SMCCF antibody
      see all

    References for Anti-MCP1 antibody [S101] (ab18678)

    ab18678 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (20%)
    Sample Rabbit Tissue lysate - whole (aorta)
    Specification aorta
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 17 2013

    La forma no glicosilada de MCP-1 migra a 11KDa. Sin embargo, el peso molecular de MCP-1 varía debido a las glicosilaciones que se dan en las células. Estas bandas que observáis a 15-20KDa deben corresponder a las formas glicosilada...

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    Thank you for your enquiry. Rabbit reactivity was tested on rabbit homogenized thoracic aorta. Dilution: Antibody concentration of 5 µg/mL (1:200) will allow visualization of 50 µg/lane of total protein. I hope this information helps. Please ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"