Anti-MCP2 antibody [35509.11] (ab10391)

Overview

  • Product nameAnti-MCP2 antibody [35509.11]
    See all MCP2 primary antibodies
  • Description
    Mouse monoclonal [35509.11] to MCP2
  • SpecificityBy ELISA, the antibody shows < 20% cross-reactivity with recombinant human MCP1 and recombinat mouse MIP1 alpha. I n addition, the antibody does not cross-react with other chemokines tested: rmC10, rhENA78, rhGRO alpha, rhGRO beta, rhGRO gamma, rmJE, rhI309, rhIL8, rhIP10, rhMIP1 alpha, rhMIP1 beta, rmMIP1 beta, rhMCP3, rhRANTES.
  • Tested applicationsSuitable for: ELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human monocyte chemotactic protein 2 (MCP2), expressed in E. coli.

  • General notesEndotoxin: < 5 ng/vial by LAL method.

Properties

Applications

Our Abpromise guarantee covers the use of ab10391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Neutralising
  • Application notesELISA: Use at a concentration of 0.5 - 1 µg/ml to detect <12.5ng/ml of recombinant human MCP2.
    Neut: Use at a concentration of 0.5 - 10 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml to detect 50ng/lane of recombinant human MCP2 under reducing and non-reducing conditions. Predicted molecular weight: 9 kDa.

    Human MCP2 is tested for its ability to neutralize the chemotactic activity of rhMCP2 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP2 that is present at a concentration just high enough to elicit a maximum response. In this bioassay 0.4ug/ml rhMCP2 was mixed with various dilutions of the antibody for 30 minutes at 22°C. After preincubation, 35ul of the antigen-antibody mixture were transferred to the lower compartment of a 96-well chemotaxis chamber. The chamber was reassembled, human monocytes were added to the top chamber and incubated at 37° for approximately 75 minutes in a humidified CO2 incubator. The chemotaxis filter was then fixed and stained. The optical density of the filter is proportional to the number of cells that migrated across the filter.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionChemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
    • Tissue specificityHighest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
    • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications
      N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ccl8 antibody
      • CCL8_HUMAN antibody
      • HC14 antibody
      • MCP-2 antibody
      • MCP-2(6-76) antibody
      • Monocyte chemoattractant protein 2 antibody
      • Monocyte chemotactic protein 2 antibody
      • SCYA10 antibody
      • SCYA8 antibody
      • Small-inducible cytokine A8 antibody
      see all

    References for Anti-MCP2 antibody [35509.11] (ab10391)

    ab10391 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply.

    All of the neutralization data was in the file that I sent you. We do guarantee our products to work as stated on the datasheet and if you experience any issue with the antibody, we will either replace or refund it f...

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    Thank you for your reply.

    Information and data regarding neutralisation withab10391 are as follows:

    The antibody was tested for its ability to neutralize the chemotactic activity of rhMCP-2. The ND50 of the antibody is defined as the ...

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    Thank you for contacting Abcam.

    Under our Abpromise, we do guarantee that ab10391 will work in neutralizing assays in mouse samples. If it does work as stated on the datasheet, then would be happy to replace or refund the antibody. You can fin...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"