Overview

  • Product name
    Anti-MCP2 antibody [35509.11]
    See all MCP2 primary antibodies
  • Description
    Mouse monoclonal [35509.11] to MCP2
  • Specificity
    By ELISA, the antibody shows < 20% cross-reactivity with recombinant human MCP1 and recombinat mouse MIP1 alpha. I n addition, the antibody does not cross-react with other chemokines tested: rmC10, rhENA78, rhGRO alpha, rhGRO beta, rhGRO gamma, rmJE, rhI309, rhIL8, rhIP10, rhMIP1 alpha, rhMIP1 beta, rmMIP1 beta, rhMCP3, rhRANTES.
  • Tested applications
    Suitable for: ELISA, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human monocyte chemotactic protein 2 (MCP2), expressed in E. coli.

  • General notes
    Endotoxin: < 5 ng/vial by LAL method.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    The antibody is purified by Protein A affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    35509.11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Neutralising
  • Application notes
    ELISA: Use at a concentration of 0.5 - 1 µg/ml to detect <12.5ng/ml of recombinant human MCP2.
    Neut: Use at a concentration of 0.5 - 10 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml to detect 50ng/lane of recombinant human MCP2 under reducing and non-reducing conditions. Predicted molecular weight: 9 kDa.

    Human MCP2 is tested for its ability to neutralize the chemotactic activity of rhMCP2 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP2 that is present at a concentration just high enough to elicit a maximum response. In this bioassay 0.4ug/ml rhMCP2 was mixed with various dilutions of the antibody for 30 minutes at 22°C. After preincubation, 35ul of the antigen-antibody mixture were transferred to the lower compartment of a 96-well chemotaxis chamber. The chamber was reassembled, human monocytes were added to the top chamber and incubated at 37° for approximately 75 minutes in a humidified CO2 incubator. The chemotaxis filter was then fixed and stained. The optical density of the filter is proportional to the number of cells that migrated across the filter.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
    • Tissue specificity
      Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
    • Sequence similarities
      Belongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications
      N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ccl8 antibody
      • CCL8_HUMAN antibody
      • HC14 antibody
      • MCP-2 antibody
      • MCP-2(6-76) antibody
      • Monocyte chemoattractant protein 2 antibody
      • Monocyte chemotactic protein 2 antibody
      • SCYA10 antibody
      • SCYA8 antibody
      • Small-inducible cytokine A8 antibody
      see all

    References

    This product has been referenced in:
    • Li X  et al. MPC1 and MPC2 expressions are associated with favorable clinical outcomes in prostate cancer. BMC Cancer 16:894 (2016). WB ; Human . Read more (PubMed: 27852261) »
    • Wozniak MA  et al. Antivirals reduce the formation of key Alzheimer's disease molecules in cell cultures acutely infected with herpes simplex virus type 1. PLoS One 6:e25152 (2011). Read more (PubMed: 22003387) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your reply.

    All of the neutralization data was in the file that I sent you. We do guarantee our products to work as stated on the datasheet and if you experience any issue with the antibody, we will either replace or refund it f...

    Read More

    Thank you for your reply.

    Information and data regarding neutralisation withab10391 are as follows:

    The antibody was tested for its ability to neutralize the chemotactic activity of rhMCP-2. The ND50 of the antibody is defined as the ...

    Read More

    Thank you for contacting Abcam.

    Under our Abpromise, we do guarantee that ab10391 will work in neutralizing assays in mouse samples. If it does work as stated on the datasheet, then would be happy to replace or refund the antibody. You can fin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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