Overview

  • Product nameAnti-MCP2 antibody
    See all MCP2 primary antibodies
  • Description
    Rabbit polyclonal to MCP2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 50-99 (SYTRITNIQC PKEAVIFKTK RGKEVCADPK ERWVRDSMKH LDQIFQNLKP) of human MCP2 (NP_005614).

  • Positive control
    • Fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • FunctionChemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
  • Tissue specificityHighest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ccl8 antibody
    • CCL8_HUMAN antibody
    • HC14 antibody
    • MCP-2 antibody
    • MCP-2(6-76) antibody
    • Monocyte chemoattractant protein 2 antibody
    • Monocyte chemotactic protein 2 antibody
    • SCYA10 antibody
    • SCYA8 antibody
    • Small-inducible cytokine A8 antibody
    see all

Anti-MCP2 antibody images

  • Anti-MCP2 antibody (ab83803) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MCP2 antibody (ab83803)

ab83803 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"