Overview

  • Product nameAnti-MCP2 antibody
    See all MCP2 primary antibodies
  • Description
    Rabbit polyclonal to MCP2
  • Tested applicationsSuitable for: WB, Neutralising, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hMCP-2 (human Macrophage/Monocyte chemotactic protein-2)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100.0 ng/ml), a concentration of 12.0 - 16.0 µg/ml of this antibody is required
IHC-P Use a concentration of 0.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP2.

Target

  • FunctionChemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
  • Tissue specificityHighest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ccl8 antibody
    • CCL8_HUMAN antibody
    • HC14 antibody
    • MCP-2 antibody
    • MCP-2(6-76) antibody
    • Monocyte chemoattractant protein 2 antibody
    • Monocyte chemotactic protein 2 antibody
    • SCYA10 antibody
    • SCYA8 antibody
    • Small-inducible cytokine A8 antibody
    see all

Anti-MCP2 antibody images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human normal gastric mucosa, staining MCP2 with ab9671 at 0.75 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Staining was detected using DAB.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human normal gastric mucosa, staining MCP2 with ab9671 at 0.75 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Staining was detected using DAB.

References for Anti-MCP2 antibody (ab9671)

This product has been referenced in:
  • Del Galdo F  et al. NF?B activation and stimulation of chemokine production in normal human macrophages by the gadolinium-based magnetic resonance contrast agent Omniscan: possible role in the pathogenesis of nephrogenic systemic fibrosis. Ann Rheum Dis 69:2024-33 (2010). IHC-P ; Human . Read more (PubMed: 20959327) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"