Overview

  • Product nameAnti-MCP3 antibody [542]
    See all MCP3 primary antibodies
  • Description
    Mouse monoclonal [542] to MCP3
  • SpecificityThis monoclonal antibody reacts with natural and recombinant human MCP 3. It does not show any cross reaction with other human cytokines or growth factors tested such as IL 1 beta, IL 8, MCAF, SAA and EGF.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment (Human).(M.W. 8.5 kDa containing 76 amino acid residues).

  • Positive control

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number542
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 8.5 kDa.
    This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3µg/well; Substrate:TMB.
    A Western blot using an antibody concentration of 0.5-2µg/mL will allow visualization of 0.1µg/lane of human MCP-3.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionChemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
    • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-C motif chemokine 7 antibody
      • Ccl7 antibody
      • CCL7_HUMAN antibody
      • Chemokine CC motif ligand 7 antibody
      • FIC antibody
      • MARC antibody
      • MCP-3 antibody
      • Monocyte chemoattractant protein 3 antibody
      • Monocyte chemotactic protein 3 antibody
      • NC28 antibody
      • RP23-350G1.4 antibody
      • SCYA6 antibody
      • SCYA7 antibody
      • Small-inducible cytokine A7 antibody
      see all

    References for Anti-MCP3 antibody [542] (ab18693)

    ab18693 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Skin)
    Loading amount 15 µg
    Specification Skin
    Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Apr 27 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"