Overview

  • Product name
    Anti-MCP3 antibody [542]
    See all MCP3 primary antibodies
  • Description
    Mouse monoclonal [542] to MCP3
  • Specificity
    This monoclonal antibody reacts with natural and recombinant human MCP 3. It does not show any cross reaction with other human cytokines or growth factors tested such as IL 1 beta, IL 8, MCAF, SAA and EGF.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment (Human).(M.W. 8.5 kDa containing 76 amino acid residues).

  • Positive control
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    542
  • Myeloma
    Sp2/0-Ag14
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 8.5 kDa.
    This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP conjugated). Suggested Capture Coating Dose: 3µg/well; Substrate:TMB.
    A Western blot using an antibody concentration of 0.5-2µg/mL will allow visualization of 0.1µg/lane of human MCP-3.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
    • Sequence similarities
      Belongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications
      O-glycosylated.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-C motif chemokine 7 antibody
      • Ccl7 antibody
      • CCL7_HUMAN antibody
      • Chemokine CC motif ligand 7 antibody
      • FIC antibody
      • MARC antibody
      • MCP-3 antibody
      • Monocyte chemoattractant protein 3 antibody
      • Monocyte chemotactic protein 3 antibody
      • NC28 antibody
      • RP23-350G1.4 antibody
      • SCYA6 antibody
      • SCYA7 antibody
      • Small-inducible cytokine A7 antibody
      see all

    References

    ab18693 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skin)
    Loading amount
    15 µg
    Specification
    Skin
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Apr 27 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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