The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MCP4.
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCP4 (Biotin) (ab83122). Using ab83122 as the detection antibody and ab110609 as the capture antibody, the detection limit for recombinant Human MCP4 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
Use a concentration of 1 - 1.5 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of MCP4 (30.0 ng/ml)
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. The detection limit for recombinant Human MCP4 is 3.0 - 4.0 ng/lane, under either reducing or non-reducing conditions.
FunctionChemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
Tissue specificityWidely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
Post-translational modificationsOne major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain.