Overview

  • Product nameAnti-MCP5 antibody
    See all MCP5 primary antibodies
  • Description
    Rabbit polyclonal to MCP5
  • Tested applicationsSuitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mMCP-5 (mouse Macrophage/Monocyte Chemotactic Protein-5).

Properties

  • FormLyophilised:Reconstitute with 200µl of sterile water.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • Isotypeunknown
  • Light chain typeunknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab9737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Neutralising
  • Application notesNeutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100.00 ng/ml), a concentration of 2.0 - 3.0 µg/ml of this antibody is required.

    ELISA:To detect mMCP-5 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.

    Western Blot:To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • Target

    • FunctionChemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
    • Tissue specificityPredominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
    • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-C motif chemokine 12 antibody
      • Ccl12 antibody
      • CCL12_MOUSE antibody
      • Macrophage/monocyte Chemotactic Protein antibody
      • MCP-1-related chemokine antibody
      • MCP-5 antibody
      • Monocyte chemoattractant protein 5 antibody
      • Monocyte chemotactic protein 5 antibody
      • OTTMUSP00000000792 antibody
      • Scya12 antibody
      • Small inducible cytokine A12 antibody
      • Small-inducible cytokine A12 antibody
      see all

    References for Anti-MCP5 antibody (ab9737)

    ab9737 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam. I am sorry that you are having trouble with ab9737. From what I have read about MCP5, it does have a predicted molecular weight of ~12kDa. I have a few questions/suggestions about the protocol that you are using: 1 - Doe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"