Overview

  • Product nameAnti-MCPIP1 antibody
    See all MCPIP1 primary antibodies
  • Description
    Rabbit polyclonal to MCPIP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human MCPIP1 aa 400-500 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q5D1E8

  • Positive control
    • This antibody gave a positive signal in the following Human tissue lysates: Heart; Spleen; Lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab70681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • FunctionHas RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19.
  • Sequence similaritiesBelongs to the ZC3H12 family.
    Contains 1 C3H1-type zinc finger.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ423B22.1 antibody
    • MCP 1 treatment-induced protein antibody
    • MCP-induced protein 1 antibody
    • MCP1-induced protein antibody
    • MCPIP 1 antibody
    • MCPIP antibody
    • Ribonuclease ZC3H12A antibody
    • RP3-423B22.1 antibody
    • ZC12A_HUMAN antibody
    • ZC3H 12A antibody
    • ZC3H12 A antibody
    • Zc3h12a antibody
    • Zinc finger CCCH domain-containing protein 12A antibody
    • Zinc finger CCCH type containing 12A antibody
    see all

Anti-MCPIP1 antibody images

  • All lanes : Anti-MCPIP1 antibody (ab70681) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Lung (Human) Tissue Lysate (ab4014)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa (possible non-specific binding),80 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab70681 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-MCPIP1 antibody (ab70681)

ab70681 has not yet been referenced specifically in any publications.

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