The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
FunctionModulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. May be an inhibitor of TERT telomerase activity. Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
Tissue specificityDetected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes.
Sequence similaritiesContains 1 FHA domain.
Developmental stageCell-cycle regulated: levels are highest early in S phase; not detectable in G2.
Cellular localizationNucleus. Nucleus > nucleolus. In microspherules in the nucleolus.