Anti-MCRS1 antibody [EPR5855] (ab124704)

Overview

  • Product nameAnti-MCRS1 antibody [EPR5855]
    See all MCRS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5855] to MCRS1
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human MCRS1.

  • Positive control
    • HeLa, HCT 116, Jurkat and LnCaP cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionModulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. May be an inhibitor of TERT telomerase activity.
      Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
    • Tissue specificityDetected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes.
    • Sequence similaritiesContains 1 FHA domain.
    • Developmental stageCell-cycle regulated: levels are highest early in S phase; not detectable in G2.
    • Cellular localizationNucleus. Nucleus > nucleolus. In microspherules in the nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 58 kDa microspherule protein antibody
      • Cell cycle regulated factor (78 kDa) antibody
      • Cell cycle regulated factor p78 antibody
      • Cell cycle-regulated factor p78 antibody
      • ICP22BP antibody
      • INO80 complex subunit J antibody
      • MCRS 1 antibody
      • MCRS 2 antibody
      • MCRS1 antibody
      • MCRS1_HUMAN antibody
      • MCRS2 antibody
      • Microspherule protein 1 antibody
      • MSP 58 antibody
      • MSP58 antibody
      • p78 antibody
      see all

    Anti-MCRS1 antibody [EPR5855] images

    • All lanes : Anti-MCRS1 antibody [EPR5855] (ab124704) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HCT 116 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : LnCaP cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-MCRS1 antibody [EPR5855] (ab124704)

    ab124704 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (colon)
    Specification colon
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Chiara Vardabasso

    Verified customer

    Submitted Nov 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"