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Product name
MCSF protein (Active)
See all MCSF products (10) ...
Protein description
Recombinant Human MCSF is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD.
Expression host
E. coli
Properties
Purity
> 95% by SDS-PAGE
Purification notes
Purity greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Biological activity
Biological Activity: The activity is determined by the dose-dependent proliferation of mouse NFS-60 cells and is typcially less than 2.0 ng/mL.
Form
Lyophilised:
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions, lyophilized from 2mg/ml.
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 5mM Sodium phosphate, 100mM Sodium chloride, pH 8
Concentration
Concentration information loading...
Additional notes
Aliquot and freeze at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Sequence notes
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Research areas
Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Immunology >> Innate Immunity >> Cytokines >> CSFs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE
Functional Studies
Application notes
FuncS: Use at an assay dependent dilution.
Biological Activity:
MCSF is fully biologically active when compared to standard. The ED50 calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 5ng/ml, corresponding to a Specific Activity of 2 x 105 IU/mg.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Post-translational
modifications
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localization
Cell membrane and Secreted > extracellular space.
Target information above from: UniProt accessionP09603
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
MCSF protein (Active) images:
Sandwich ELISA - MCSF protein (ab62015)
Standard curve for MCSF (Analyte: ab62015); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1µg/ml.
Other-MCSF protein (Active)(ab62015)
ab62015 used in Western Blot. Figure: 1 ug run under (+) reducing conditions and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human M-CSF is a homodimer with a total predicted MW of 36.8 kDa.
References for MCSF protein (Active) (ab62015)
ab62015 has not yet been referenced specifically in any publications.
Publishing research using ab62015? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"