Overview

  • Product nameAnti-MCSF Receptor antibody
    See all MCSF Receptor primary antibodies
  • Description
    Rabbit polyclonal to MCSF Receptor
  • Specificityab59231 detects endogenous levels of total GM-CSF Receptor alpha protein.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human MCSF Receptor alpha around the phosphorylation site of tyrosine 809 (S-N-YP-V-V).

  • Positive control
    • 293 cell extracts treated with LPS (100ng/ml, 30mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab59231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 46 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionProtein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
  • Tissue specificityExpressed in bone marrow and in differentiated blood mononuclear cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C FMS antibody
    • CD 115 antibody
    • CD115 antibody
    • CD115 antigen antibody
    • CFMS antibody
    • Colony stimulating factor 1 receptor antibody
    • Colony stimulating factor I receptor antibody
    • CSF 1 R antibody
    • CSF 1R antibody
    • CSF-1 receptor antibody
    • CSF-1-R antibody
    • CSF1 R antibody
    • CSF1R antibody
    • CSF1R_HUMAN antibody
    • CSFR antibody
    • EC 2.7.10.1 antibody
    • FIM 2 antibody
    • FIM2 antibody
    • FMS antibody
    • FMS proto oncogene antibody
    • FMS protooncogene antibody
    • HDLS antibody
    • M-CSF Receptor antibody
    • M-CSF-R antibody
    • Macrophage colony stimulating factor 1 receptor antibody
    • Macrophage colony stimulating factor I receptor antibody
    • Macrophage colony-stimulating factor 1 receptor antibody
    • McDonough feline sarcoma viral (v fms) oncogene homolog antibody
    • MCSFR antibody
    • Oncogen FMS antibody
    • Proto-oncogene c-Fms antibody
    • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly antibody
    see all

Anti-MCSF Receptor antibody images

  • All lanes : Anti-MCSF Receptor antibody (ab59231) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with LPS (100ng/ml, 30mins)
    Lane 2 : 293 cell extracts treated with LPS (100ng/ml, 30mins) with immunising peptide


    Predicted band size : 46 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Ab59231 staining Human placenta. Staining is localised to the cell membrane.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-MCSF Receptor antibody (ab59231)

ab59231 has not yet been referenced specifically in any publications.

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