Overview

  • Product name
    Anti-MCSF Receptor antibody [SP211]
    See all MCSF Receptor primary antibodies
  • Description
    Rabbit monoclonal [SP211] to MCSF Receptor
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human MCSF Receptor. The exact sequence is proprietary.
    Database link: P07333

  • Positive control
    • Human colon, lung, liver, skin, tonsil and skin squamous cell carcinoma tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab183316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function
    Protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
  • Tissue specificity
    Expressed in bone marrow and in differentiated blood mononuclear cells.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C FMS antibody
    • CD 115 antibody
    • CD115 antibody
    • CD115 antigen antibody
    • CFMS antibody
    • Colony stimulating factor 1 receptor antibody
    • Colony stimulating factor I receptor antibody
    • CSF 1 R antibody
    • CSF 1R antibody
    • CSF-1 receptor antibody
    • CSF-1-R antibody
    • CSF1 R antibody
    • CSF1R antibody
    • CSF1R_HUMAN antibody
    • CSFR antibody
    • EC 2.7.10.1 antibody
    • FIM 2 antibody
    • FIM2 antibody
    • FMS antibody
    • FMS proto oncogene antibody
    • FMS protooncogene antibody
    • HDLS antibody
    • M-CSF Receptor antibody
    • M-CSF-R antibody
    • Macrophage colony stimulating factor 1 receptor antibody
    • Macrophage colony stimulating factor I receptor antibody
    • Macrophage colony-stimulating factor 1 receptor antibody
    • McDonough feline sarcoma viral (v fms) oncogene homolog antibody
    • MCSFR antibody
    • Oncogen FMS antibody
    • Proto-oncogene c-Fms antibody
    • V-FMS McDonough feline sarcoma viral oncogen homolog, formerly antibody
    see all

Images

  • Immunohistochemical analysis of Human tonsil tissue labeling MCSF Receptor using ab183316 at a 1/100 dilution

  • Immunohistochemical analysis of Human skin tissue labeling MCSF Receptor using ab183316 at a 1/100 dilution

  • Immunohistochemical analysis of Human skin squamous cell carcinoma tissue labeling MCSF Receptor using ab183316 at a 1/100 dilution

  • Immunohistochemical analysis of Human colon tissue labeling MCSF Receptor using ab183316 at a 1/100 dilution

  • Immunohistochemical analysis of Human lung tissue labeling MCSF Receptor using ab183316 at a 1/100 dilution

  • Immunohistochemical analysis of Human liver tissue labeling MCSF Receptor using ab183316 at a 1/100 dilution

References

This product has been referenced in:
  • Guo X  et al. Hypoxia promotes glioma-associated macrophage infiltration via periostin and subsequent M2 polarization by upregulating TGF-beta and M-CSFR. Oncotarget 7:80521-80542 (2016). IHC, ICC/IF ; Human . Read more (PubMed: 27602954) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (tibia)
Antigen retrieval step
Other
Permeabilization
No
Specification
tibia
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Feb 29 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Na-citrate, pH6.0
Permeabilization
No
Specification
Bone
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 23 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Lymph node)
Permeabilization
No
Specification
Lymph node
Blocking step
Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Sep 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.0
Permeabilization
No
Specification
Lung
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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