Anti-MCT7 antibody (ab21947)

Overview

  • Product name
  • Description
    Mouse polyclonal to MCT7
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein:

    TFGVFFNDLMDSFNESNSR

    , corresponding to amino acids 44/62 of Human MCT7

  • General notes
    Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab21947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • Relevance
    MCT7 is a proton linked monocarboxylate transporter. It catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. MCT7 belongs to the major facilitator superfamily.
  • Cellular localization
    Cell membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • Breast cancer protein BCY5 antibody
    • MCT 6 antibody
    • MCT 7 antibody
    • Monocarboxylate transporter 6 antibody
    • Monocarboxylate transporter 7 antibody
    • SLC16A6 antibody
    • Solute carrier family 16 (monocarboxylic acid transporters) member 6 antibody
    • Solute carrier family 16 member 6 antibody
    see all

Anti-MCT7 antibody images

  • All lanes : Anti-MCT7 antibody (ab21947) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 57 kDa

References for Anti-MCT7 antibody (ab21947)

ab21947 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

Unfortunately, the blocking peptide for this ab21947 is not available. I am afraid that we also do not have any other peptides or proteins for MCT7 that could be used as a blocking peptide.

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Endometrium)
Specification
Endometrium
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Permeabilization
No
Blocking step
Horse serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Jul 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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