Overview

  • Product nameAnti-MCTP1 antibody
    See all MCTP1 primary antibodies
  • Description
    Rabbit polyclonal to MCTP1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 720-769 (LVWGINKFTK KLRSPYAIDN NELLDFLSRV PSDVQVVQYQ ELKPDPSHSP) of Human MCTP1 (NP_001002796)

  • Positive control
    • HeLa cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab83673 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab83673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceMCTP1 binds calcium via the C2 domains in the absence of phospholipids. There are 5 named isoforms.
  • Cellular localizationMembrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • 2810465F10Rik antibody
    • FLJ22344 antibody
    • MCTP 1 antibody
    • Multiple C2 and transmembrane domain containing protein 1 antibody
    • Multiple C2 domains transmembrane 1 antibody
    • Multiple C2 domains with two transmembrane regions 1 antibody
    • OTTMUSP00000031345 antibody
    • RGD1305199 antibody
    see all

Anti-MCTP1 antibody images

  • Anti-MCTP1 antibody (ab83673) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 37 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • ab83673 at 5 µg/ml staining MCTP1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab83673 at 5µg/ml staining MCTP1 in Human Liver tissue by Immunohistochemistry (Formali/PFA-fixed paraffin-embedded sections).

References for Anti-MCTP1 antibody (ab83673)

ab83673 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I have not seen that we have tested or have any additional data regarding the dimerization of this protein or which reducing agents might be more effective. We generally use a 2x Laemmli buffer with 4% SDS, or use o...

Read More

Thank you for contacting Abcam.

I am sorry to hear of the difficulties that you have been experiencing using these products. Would you mind sending me the details of your protocol and any steps that you may have changes while troubleshooting...

Read More

DISCOUNT CODE: *
Expiration date: 27th Nov 2012

I am very pleased to hear you would like to accept our offer and test ab83673 in ICC/IF. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, ple...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"