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Product name Anti-MCU antibody
Rabbit polyclonal to MCU
Tested applications Suitable for:
WB, IHC-P, ICC/IF more details
Predicted to work with:
Recombinant fragment: HHRTVHQRIA SWQNLGAVYC STVVPSDDVT VVYQNGLPVI SVRLPSRRER CQFTLKPISD SVGVFLRQLQ EEDRGIDRVA IYSPDGVRVA ASTGIDLLLL DDFKLV, corresponding to N terminal amino acids 47-152 of Human MCU (Uniprot ID = Q8NE86)
Human duodenum tissue. Human cell line U-2 OS.
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer pH: 7.20 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml. Recommended fixative: PFA/Triton X-100
Sequence similarities Belongs to the CCDC109 family.
Cellular localization Membrane.
Information by UniProt
Coiled-coil domain-containing protein 109A antibody
Mitochondrial Calcium Uniporter antibody
Anti-MCU antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab121499)
Immunohistochemical analysis of paraffin-embedded Human duodenum labeling MCU in glandular cells with ab121499 at 1/50 dilution. Heat mediated antigen retrieval with citrate buffer pH 6 was performed.
Immunocytochemistry/ Immunofluorescence - (ab121499)
Immunofluorescent staining of Human cell line U-2 OS (fixed and permeabilized in PFA/Triton X-100) labeling MCU with ab121499 at 4µg/ml.
References for Anti-MCU antibody (ab121499)
This product has been referenced in:
et al. Mitochondrial Ca2+-dependent NLRP3 activation exacerbates the Pseudomonas aeruginosa-driven inflammatory response in cystic fibrosis. Nat Commun 6:6201 (2015).
Read more (PubMed: 25648527) »
et al. miR-181c Regulates the Mitochondrial Genome, Bioenergetics, and Propensity for Heart Failure In Vivo. PLoS One 9:e96820 (2014).
Read more (PubMed: 24810628) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"