Overview

  • Product nameAnti-MD2 antibody [9F1B1]
    See all MD2 primary antibodies
  • Description
    Mouse monoclonal [9F1B1] to MD2
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein corresponding to amino acids 2 to 160 of human MD2

  • Positive control
    • MD2 recombinant protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab73550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionCooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.
  • Post-translational
    modifications
    N-glycosylated; high-mannose.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESOP 1 antibody
    • ESOP-1 antibody
    • ESOP1 antibody
    • LY 96 antibody
    • Ly-96 antibody
    • LY96 antibody
    • LY96_HUMAN antibody
    • Lymphocyte antigen 96 antibody
    • md 2 antibody
    • MD 2 protein antibody
    • MD2 protein antibody
    • Myeloid differentiation protein 2 antibody
    • Protein MD 2 antibody
    • Protein MD-2 antibody
    • Protein MD2 antibody
    see all

Anti-MD2 antibody [9F1B1] images

  • Immunofluorescence of MD2 in Human spleen tissue with ab73550 at 20 ug/mL.
  • All lanes : Anti-MD2 antibody [9F1B1] (ab73550) at 1 µg/ml

    Lane 1 : MD2 recombinant protein at 0.025 µg
    Lane 2 : MD2 recombinant protein at 0.125 µg


    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • ab73550 at 2.5 µg/ml staining MD2 in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

References for Anti-MD2 antibody [9F1B1] (ab73550)

This product has been referenced in:
  • Hiratsuka S  et al. Primary tumours modulate innate immune signalling to create pre-metastatic vascular hyperpermeability foci. Nat Commun 4:1853 (2013). Mouse . Read more (PubMed: 23673638) »

See 1 Publication for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your reply. Our products arecovered by our Abpromise guarantee. If these are used according to the specifications on the datasheet, e.g. species and applications listed, we offer not only scientific support but replacement or refund sh...

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Thank you for your reply.

In light of this information I would be very happy to replace this product with another anti-MD2 antibody provided you have ordered within the last six months. We do have many products which are known to react in ...

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Thank you for your reply. I am currently trying to get the control peptide to you. This may take a few days as it needs to be transferred and processed. I will keep you informed of this as I learn more. I was also able to find record of the lab's...

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Thank you for contacting us. The only data that I have found in western blot is with the MD2 recombinant protein. This product has been shown to react with mouse in IHC. I believe it is the cross reactivity information referenced on our datasheet. I...

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Thank you for contacting us.

I am sorry to hear that you feel the product is not performing as it should and would like to help you with this problem.

In order to help determine what may be the problem here, would you be able to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"