• Product nameAnti-MDA5 antibody
    See all MDA5 primary antibodies
  • Description
    Rabbit polyclonal to MDA5
  • SpecificityDetects endogenous levels of total MDA5 protein.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized peptide derived from C-terminal of human MDA5.

  • Positive control
    • Extracts from Jurkat cells



Our Abpromise guarantee covers the use of ab69983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 24886842ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa).
ELISA 1/40000.
ICC/IF Use a concentration of 1 µg/ml.


  • FunctionRNA helicase that, through its ATP-dependent unwinding of RNA, may function to promote message degradation by specific RNases. Seems to have growth suppressive properties. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). ATPase activity is specifically induced by dsRNA. Essential for the production of interferons in response to picornaviruses.
  • Tissue specificityWidely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung.
  • Involvement in diseaseGenetic variation in IFIH1 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19) [MIM:610155]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Note=IFIH1 is the CADM-140 autoantigen, involved in clinically amyopathic dermatomyositis (CADM). This is a chronic inflammatory disorder that shows typical skin manifestations of dermatomyositis but has no or little evidence of clinical myositis. Anti-CADM-140 antibodies appear to be specific to dermatomyositis, especially CADM. Patients with anti-CADM-140 antibodies frequently develop life-threatening acute progressive interstitial lung disease (ILD).
  • Sequence similaritiesBelongs to the helicase family.
    Contains 2 CARD domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    During apoptosis, processed into 3 cleavage products. The helicase-containing fragment, once liberated from the CARD domains, translocate from the cytoplasm to the nucleus. The processed protein significantly sensitizes cells to DNA degradation.
  • Cellular localizationCytoplasm. Nucleus. May be found in the nucleus, during apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADM-140 autoantigen antibody
    • Clinically amyopathic dermatomyositis autoantigen 140 kDa antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide antibody
    • DEAD/H box polypeptide antibody
    • Helicard antibody
    • Helicase with 2 CARD domains antibody
    • Hlcd antibody
    • IDDM 19 antibody
    • IDDM19 antibody
    • IFIH 1 antibody
    • Ifih1 antibody
    • IFIH1_HUMAN antibody
    • Interferon induced helicase C domain containing protein 1 antibody
    • interferon induced with helicase C domain 1 antibody
    • Interferon induced with helicase C domain protein 1 antibody
    • Interferon-induced helicase C domain-containing protein 1 antibody
    • Interferon-induced with helicase C domain protein 1 antibody
    • MDA 5 antibody
    • MDA-5 antibody
    • Melanoma differentiation associated protein 5 antibody
    • Melanoma differentiation-associated gene 5 antibody
    • Melanoma differentiation-associated protein 5 antibody
    • MGC133047 antibody
    • Murabutide down regulated protein antibody
    • Murabutide down-regulated protein antibody
    • RH 116 antibody
    • RH116 antibody
    • RIG I like receptor 2 antibody
    • RLR 2 antibody
    • RNA helicase DEAD box protein 116 antibody
    • RNA helicase-DEAD box protein 116 antibody
    see all

Anti-MDA5 antibody images

  • All lanes : Anti-MDA5 antibody (ab69983) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells (5-30µg)
    Lane 2 : Extracts from Jurkat cells (5-30µg) plus immunising peptide (5-10µg)

    Predicted band size : 117 kDa
    Observed band size : 117 kDa
  • Ab69983 (2µg/ml) staining MDA5 in human thyroid gland using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and moderate nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • ICC/IF image of ab69983 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69983, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MDA5 antibody (ab69983)

This product has been referenced in:
  • Tengroth L  et al. Functional effects of Toll-like receptor (TLR)3, 7, 9, RIG-I and MDA-5 stimulation in nasal epithelial cells. PLoS One 9:e98239 (2014). IHC-P, Flow Cyt ; Human . Read more (PubMed: 24886842) »
  • Yu L  et al. Pattern recognition receptor-initiated innate antiviral response in mouse adipose cells. Immunol Cell Biol N/A:N/A (2013). Mouse . Read more (PubMed: 24165978) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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Submitted Nov 10 2014

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The lysate preparation is a standard procedure which requires RIPA buffer (containing a cocktail of proteinase inhibitors). The composition of this lysate buffer can be found under the Protocol se...

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