Overview

  • Product name
  • Description
    Chicken polyclonal to MDC
  • Specificity
    By Immunoblotting and ELISA the antibody shows approximately 20% cross-reactivity with recombinant human MPIF1, 10% cross-reactivity with recombinant human MIP1 delta and 1% cross-reactivity with recombinant mouse MDC, recombinant human HCC-1, recombinant human I-309, recombinant mouse MIP1 alpha, recombinant viral MIP1, recombinant mouse MIP1 beta and recombinant mouse MIP1 gamma.
  • Tested applications
    Suitable for: ELISA, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant human MDC, expressed in E. coli.

  • General notes

    Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.

    MDC is a member of the beta (CC) chemokine family and induces chemotaxis or calcium mobilization in dendritic cells, IL-2 activated cells and activated T lymphocytes. MDC, also named stimulated T cell chemotactic protein (STCP-1), was isolated from clones of monocyted-derived macrophages and binds to CC chemokine receptor 4 (CCR4). MDC shows less than 35% identity to other C-C chemokine family members. It is expressed in dendritic cells, macrophages and activated monocytes. Expression is also detected in the tissues of thymus, lymph node and appendix. NH2- terminally truncated forms of natural MDC were isolated from a CD8+ T cell clone and have been reported to inhibit HIV-1 infection.

    WB: Working concentrations determined using human MDC at 5 ng/lane and 20 ng/ml under non-reducing and reducing conditions, respectively. To measure the ability of the antibody to neutralize the bioactivity of human MDC, recombinant human MDC was incubated with various concentrations of the antibody for 30 minutes in a microwell plate. Following preincubation, 75 µl of cytokine-antibody solution (containing antibody at concentrations of 0.01- 100 µg/ml and recombinant human MDC at 0.05 µg/ml)was transferred to the lower compartment of a 96-well chemotaxis chamber. The chamber was assembled using a PVP-free polycarbonate filter and 2 x 106 cells/well were added to the top chamber. After the plate was incubated at 37°C for 3 hours in a humidified CO2 incubator, the cells that migrated through to the lower chamber were stained with Alamar blue and the relative fluorescence was measured. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The specific IgY antibody is purified using human MDC affinity chromatography.
  • Primary antibody notes
    MDC is a member of the beta (CC) chemokine family and induces chemotaxis or calcium mobilization in dendritic cells, IL-2 activated cells and activated T lymphocytes. MDC, also named stimulated T cell chemotactic protein (STCP-1), was isolated from clones of monocyted-derived macrophages and binds to CC chemokine receptor 4 (CCR4). MDC shows less than 35% identity to other C-C chemokine family members. It is expressed in dendritic cells, macrophages and activated monocytes. Expression is also detected in the tissues of thymus, lymph node and appendix. NH2- terminally truncated forms of natural MDC were isolated from a CD8+ T cell clone and have been reported to inhibit HIV-1 infection.
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 - 1 µg/ml.

Use at a concentration of 0.5 - 1 µg/ml to detect a limit of 0.3 ng/well of recombinant human MDC.

Neutralising Use a concentration of 0.5 - 1.5 µg/ml.

This working concentration of Anti-Human MDC will neutralize 50% of the bioactivity due to 50 ng/ml recombinant human MDC.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10.5 kDa.

Target

  • Function
    May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
  • Tissue specificity
    Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A 152E5.1 antibody
    • ABCD 1 antibody
    • ABCD1 antibody
    • C C motif chemokine 22 antibody
    • CC chemokine STCP 1 antibody
    • CC chemokine STCP-1 antibody
    • ccl 22 antibody
    • Ccl22 antibody
    • CCL22_HUMAN antibody
    • Chemokine (C C motif) ligand 22 antibody
    • DC/B CK antibody
    • DCBCK antibody
    • Macrophage-derived chemokine antibody
    • MDC antibody
    • MDC(1-69) antibody
    • MDC(7-69) antibody
    • MGC34554 antibody
    • SCYA22 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 22 antibody
    • Small inducible cytokine subfamily A, member 22 antibody
    • Small-inducible cytokine A22 antibody
    • STCP 1 antibody
    • STCP1 antibody
    • Stimulated T cell chemotactic protein 1 antibody
    • Stimulated T-cell chemotactic protein 1 antibody
    see all

References

ab10379 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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