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Purified recombinant human MDC, expressed in E. coli.
Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.
MDC is a member of the beta (CC) chemokine family and induces chemotaxis or calcium mobilization in dendritic cells, IL-2 activated cells and activated T lymphocytes. MDC, also named stimulated T cell chemotactic protein (STCP-1), was isolated from clones of monocyted-derived macrophages and binds to CC chemokine receptor 4 (CCR4). MDC shows less than 35% identity to other C-C chemokine family members. It is expressed in dendritic cells, macrophages and activated monocytes. Expression is also detected in the tissues of thymus, lymph node and appendix. NH2- terminally truncated forms of natural MDC were isolated from a CD8+ T cell clone and have been reported to inhibit HIV-1 infection.
WB: Working concentrations determined using human MDC at 5 ng/lane and 20 ng/ml under non-reducing and reducing conditions, respectively. To measure the ability of the antibody to neutralize the bioactivity of human MDC, recombinant human MDC was incubated with various concentrations of the antibody for 30 minutes in a microwell plate. Following preincubation, 75 µl of cytokine-antibody solution (containing antibody at concentrations of 0.01- 100 µg/ml and recombinant human MDC at 0.05 µg/ml)was transferred to the lower compartment of a 96-well chemotaxis chamber. The chamber was assembled using a PVP-free polycarbonate filter and 2 x 106 cells/well were added to the top chamber. After the plate was incubated at 37°C for 3 hours in a humidified CO2 incubator, the cells that migrated through to the lower chamber were stained with Alamar blue and the relative fluorescence was measured. The ND50 is the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response.
Our Abpromise guarantee covers the use of ab10379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 - 1 µg/ml.
Use at a concentration of 0.5 - 1 µg/ml to detect a limit of 0.3 ng/well of recombinant human MDC.
|Neutralising||Use a concentration of 0.5 - 1.5 µg/ml.
This working concentration of Anti-Human MDC will neutralize 50% of the bioactivity due to 50 ng/ml recombinant human MDC.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10.5 kDa.|
ab10379 has not yet been referenced specifically in any publications.