Overview

  • Product name
  • Description
    Rabbit polyclonal to MDC
  • Tested applications
    Suitable for: Sandwich ELISA, Neutralising, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hMDC(human Macrophage-Derived Chemokine)

Properties

  • Form
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

This antibody used as the capture antibody can be paired with ab83129 as the detection antibody allowing the detection of at least 0.2 - 0.4 ng/well of recombinant hMDC.

Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hMDC (100 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required.
WB Use a concentration of 0.1 - 0.2 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 19318474

Target

  • Function
    May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
  • Tissue specificity
    Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A 152E5.1 antibody
    • ABCD 1 antibody
    • ABCD1 antibody
    • C C motif chemokine 22 antibody
    • CC chemokine STCP 1 antibody
    • CC chemokine STCP-1 antibody
    • ccl 22 antibody
    • Ccl22 antibody
    • CCL22_HUMAN antibody
    • Chemokine (C C motif) ligand 22 antibody
    • DC/B CK antibody
    • DCBCK antibody
    • Macrophage-derived chemokine antibody
    • MDC antibody
    • MDC(1-69) antibody
    • MDC(7-69) antibody
    • MGC34554 antibody
    • SCYA22 antibody
    • Small inducible cytokine subfamily A (Cys Cys) member 22 antibody
    • Small inducible cytokine subfamily A, member 22 antibody
    • Small-inducible cytokine A22 antibody
    • STCP 1 antibody
    • STCP1 antibody
    • Stimulated T cell chemotactic protein 1 antibody
    • Stimulated T-cell chemotactic protein 1 antibody
    see all

References

This product has been referenced in:
  • Liang D  et al. Activated STING enhances Tregs infiltration in the HPV-related carcinogenesis of tongue squamous cells via the c-jun/CCL22 signal. Biochim Biophys Acta 1852:2494-503 (2015). Read more (PubMed: 26303640) »
  • Döring C  et al. A novel immunohistochemical classifier to distinguish Hodgkin lymphoma from ALK anaplastic large cell lymphoma. Mod Pathol N/A:N/A (2014). Read more (PubMed: 24633193) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Colorectal)
Permeabilization
Yes - 1x PBS with 0.3% triton-x-100
Specification
Colorectal
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 08 2015



ab9675 and ab9847 are not conjugated. If you would like to conjugate them to fluorochromes, we have easy kits which would allow you to do this. Please see: http://www.abcam.com/easylink

You will find our recommended intracellular ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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