Overview

  • Product nameAnti-MDC antibody [EPR1362]
    See all MDC primary antibodies
  • Description
    Rabbit monoclonal [EPR1362] to MDC
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Mouse MDC aa 1 to the C-terminus.
    Database link: O88430

  • Positive control
    • MDC full-length recombinant protein; Mouse colon tissue and mouse spleen tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR1362
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 10 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).
  • Application notesIs unsuitable for ICC/IF or IP.
  • Target

    • FunctionMay play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
    • Tissue specificityHighly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
    • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
    • Post-translational
      modifications
      The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • A 152E5.1 antibody
      • ABCD 1 antibody
      • ABCD1 antibody
      • C C motif chemokine 22 antibody
      • CC chemokine STCP 1 antibody
      • CC chemokine STCP-1 antibody
      • ccl 22 antibody
      • Ccl22 antibody
      • CCL22_HUMAN antibody
      • Chemokine (C C motif) ligand 22 antibody
      • DC/B CK antibody
      • DCBCK antibody
      • Macrophage-derived chemokine antibody
      • MDC antibody
      • MDC(1-69) antibody
      • MDC(7-69) antibody
      • MGC34554 antibody
      • SCYA22 antibody
      • Small inducible cytokine subfamily A (Cys Cys) member 22 antibody
      • Small inducible cytokine subfamily A, member 22 antibody
      • Small-inducible cytokine A22 antibody
      • STCP 1 antibody
      • STCP1 antibody
      • Stimulated T cell chemotactic protein 1 antibody
      • Stimulated T-cell chemotactic protein 1 antibody
      see all

    Anti-MDC antibody [EPR1362] images

    • Anti-MDC antibody [EPR1362] (ab124768) at 1/1000 dilution + Recombinant full-length MDC protein at 0.005 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 10 kDa
    • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MDC with ab124768 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling MDC with ab124768 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

    References for Anti-MDC antibody [EPR1362] (ab124768)

    ab124768 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124768.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"