Anti-MDC1 antibody (ab11169)
Key features and details
- Rabbit polyclonal to MDC1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MDC1 antibody
See all MDC1 primary antibodies -
Description
Rabbit polyclonal to MDC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human MDC1 aa 450-550.
Database link: 9656 -
Positive control
- Tested with HeLa cells and HEK 293 cells.
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General notes
In response to recent feedback about mouse species we no longer guarantee this anymore. Please do contact our scientific support team for more information.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (3) |
Use at an assay dependent concentration.
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WB | (4) |
1/500 - 1/2500. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2500. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa). |
Target
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Function
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Contains 2 BRCT domains.
Contains 1 FHA domain. -
Domain
Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX. -
Post-translational
modificationsPhosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. -
Cellular localization
Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 9656 Human
- GenBank: NP_055456 Human
- Omim: 607593 Human
- SwissProt: Q14676 Human
- Unigene: 653495 Human
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Alternative names
- Homologue to Drosophila photoreceptor protein calphotin antibody
- MDC 1 antibody
- Mdc1 antibody
see all
Images
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Samples:
A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) ab11169 used at the indicated concentrations for WB. B) ab11170 (column labelled BL579) and ab11171 (column labelled BL580) (a ) used at 3.3 mcg/mg lysate for IP followed by WB using ab11169 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min. Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) ab11169 used at the indicated concentrations for WB. B) ab11170 (column labelled BL579) and ab11171 (column labelled BL580) (a ) used at 3.3 mcg/mg lysate for IP followed by WB using ab11169 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (42)
ab11169 has been referenced in 42 publications.
- Qiao G et al. LIGHT enhanced bispecific antibody armed T-cells to treat immunotherapy resistant colon cancer. Oncogene 41:2054-2068 (2022). PubMed: 35177811
- Giacosa S et al. Cooperative Blockade of CK2 and ATM Kinases Drives Apoptosis in VHL-Deficient Renal Carcinoma Cells through ROS Overproduction. Cancers (Basel) 13:N/A (2021). PubMed: 33540838
- Komurková D et al. G-Quadruplex Structures Colocalize with Transcription Factories and Nuclear Speckles Surrounded by Acetylated and Dimethylated Histones H3. Int J Mol Sci 22:N/A (2021). PubMed: 33671470
- Etourneaud L et al. Lamin B1 sequesters 53BP1 to control its recruitment to DNA damage. Sci Adv 7:N/A (2021). PubMed: 34452908
- Campillo-Marcos I et al. Lysine Methyltransferase Inhibitors Impair H4K20me2 and 53BP1 Foci in Response to DNA Damage in Sarcomas, a Synthetic Lethality Strategy. Front Cell Dev Biol 9:715126 (2021). PubMed: 34540832