Overview

  • Product nameAnti-MDC1 antibody
    See all MDC1 primary antibodies
  • Description
    Rabbit polyclonal to MDC1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    RQESDKNGESASPERDRE

    , corresponding to amino acids 871-888 of Human MDC1.

  • Positive control
    • Jurkat whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab13858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 220 kDa (predicted molecular weight: 250 kDa).

Target

  • FunctionRequired for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • Tissue specificityHighly expressed in testis.
  • Sequence similaritiesContains 2 BRCT domains.
    Contains 1 FHA domain.
  • DomainTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • Post-translational
    modifications
    Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • Cellular localizationNucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Anti-MDC1 antibody images



  • Predicted band size : 250 kDa


    Western blot analysis of MDC1 in 30 ugs of Jurkat cell lysate using ab 13858 at 1/1000 dilution.

References for Anti-MDC1 antibody (ab13858)

This product has been referenced in:
  • Ismail IA  et al. Genistein-induced neuronal apoptosis and G2/M cell cycle arrest is associated with MDC1 up-regulation and PLK1 down-regulation. Eur J Pharmacol 575:12-20 (2007). WB ; Human . Read more (PubMed: 17706963) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"