Overview

  • Product nameAnti-MDC1 antibody
    See all MDC1 primary antibodies
  • Description
    Rabbit polyclonal to MDC1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding a region between residues 1375 and 1425 of Human MDC1 (NP_055456.1)

  • Positive control
    • Human skin tumour tissue. Human breast adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab84079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
    • Tissue specificityHighly expressed in testis.
    • Sequence similaritiesContains 2 BRCT domains.
      Contains 1 FHA domain.
    • DomainTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
    • Post-translational
      modifications
      Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
    • Cellular localizationNucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
    • Information by UniProt
    • Database links
    • Alternative names
      • Homologue to Drosophila photoreceptor protein calphotin antibody
      • MDC 1 antibody
      • Mdc1 antibody
      • MDC1_HUMAN antibody
      • Mediation of DNA damage checkpoint 1 antibody
      • Mediator of DNA damage checkpoint 1 antibody
      • Mediator of DNA damage checkpoint protein 1 antibody
      • NFBD 1 antibody
      • NFBD1 antibody
      • Nuclear factor with BRCT domains 1 antibody
      • Nuclear Factor with BRCT Domains Protein 1 antibody
      see all

    Anti-MDC1 antibody images

    • Detection of Human MDC1 by Immunohistochemistry.
      Sample: FFPE section of Human skin tumour.
      Antibody: ab84079 used at a dilution of 1/250.
      Detection: DAB staining using anti Rabbit IHC antibody at a dilution of 1/100.
    • Detection of Human MDC1 by Immunohistochemistry.
      Sample: FFPE section of Human breast adenocarcinoma.
      Antibody: ab84079 used at a dilution of 1/250.
      Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.

    References for Anti-MDC1 antibody (ab84079)

    ab84079 has not yet been referenced specifically in any publications.

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