Overview

  • Product nameAnti-MDC1 antibody [MDC1-50]
    See all MDC1 primary antibodies
  • Description
    Mouse monoclonal [MDC1-50] to MDC1
  • Tested applicationsSuitable for: WB, IP, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant fragment, corresponding to amino acids 2-200 of Human MDC1

  • Positive control
    • WB: total cell extract of G361 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purification notesThis antibody is a purified Mouse Immunoglobulin.
  • ClonalityMonoclonal
  • Clone numberMDC1-50
  • MyelomaNS1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 226 kDa.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionRequired for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
  • Tissue specificityHighly expressed in testis.
  • Sequence similaritiesContains 2 BRCT domains.
    Contains 1 FHA domain.
  • DomainTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
  • Post-translational
    modifications
    Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
  • Cellular localizationNucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homologue to Drosophila photoreceptor protein calphotin antibody
    • MDC 1 antibody
    • Mdc1 antibody
    • MDC1_HUMAN antibody
    • Mediation of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint 1 antibody
    • Mediator of DNA damage checkpoint protein 1 antibody
    • NFBD 1 antibody
    • NFBD1 antibody
    • Nuclear factor with BRCT domains 1 antibody
    • Nuclear Factor with BRCT Domains Protein 1 antibody
    see all

Anti-MDC1 antibody [MDC1-50] images

  • ab50003 staining MDC1 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using NP40, blocked with 3% BSA for 3 hours at 25°C and then incubated with ab50003 at a 1/200 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated sheep anti-mouse IgG polyclonal used at a 1/500 dilution.

    MDC1 appears as green foci, the nucleus has been stained with DAPI (blue).

    See Abreview

References for Anti-MDC1 antibody [MDC1-50] (ab50003)

This product has been referenced in:
  • Gao M  et al. Ago2 facilitates Rad51 recruitment and DNA double-strand break repair by homologous recombination. Cell Res 24:532-41 (2014). WB ; Human . Read more (PubMed: 24662483) »
  • Henriksson S  et al. The scaffold protein WRAP53ß orchestrates the ubiquitin response critical for DNA double-strand break repair. Genes Dev 28:2726-38 (2014). Human . Read more (PubMed: 25512560) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (smooth muscle cells)
Specification smooth muscle cells
Fixative Acetone
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Formaldehyde
Permeabilization Yes - NP40
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Andrew Cobb

Verified customer

Submitted Mar 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"