The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 µg/ml. Detects a band of approximately 287 kDa (predicted molecular weight: 227 kDa).
FunctionRequired for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
DomainTandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX.
Post-translational modificationsPhosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint.
Cellular localizationNucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks.
Homologue to Drosophila photoreceptor protein calphotin antibody
MDC 1 antibody
Mediation of DNA damage checkpoint 1 antibody
Mediator of DNA damage checkpoint 1 antibody
Mediator of DNA damage checkpoint protein 1 antibody
NFBD 1 antibody
Nuclear factor with BRCT domains 1 antibody
Nuclear Factor with BRCT Domains Protein 1 antibody
Anti-MDC1 (phospho T4) antibody images
Immunocytochemistry/ Immunofluorescence - Anti-MDC1 (phospho T4) antibody (ab35967)This image is courtesy of an anonymous Abreview
ab35967 staining MDC1 (phospho T4) in HeLa cells (untreated or treated with 10 Gy IR) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 5% BSA for 1 hour. Samples were incubated with primary antibody (1/200 in 5% BSA) for 1 hour. A FITC-conjugated donkey anti-rabbit IgG polyclonal (1/150) was used as the secondary antibody.
This antibody gave a positive result in ELISA against the immunizing peptide (blue line, ab36513). It gave a negative result in ELISA against the non-modified equivalent peptide (yellow line, ab36514). This indicates that it is specific for the modified peptide.
References for Anti-MDC1 (phospho T4) antibody (ab35967)
has not yet been referenced specifically in any publications.
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