Overview

  • Product nameAnti-MDFIC antibody
    See all MDFIC primary antibodies
  • Description
    Rabbit polyclonal to MDFIC
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the N terminal region of Human MDFIC (NP_951038.1).

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab93224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 37 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMDFIC is a cellular factor capable of modulating the expression of cellular and viral promoters. Its synthesis is controlled at the translational level by two different codons, an ATG and an upstream non-ATG translational initiator, allowing the production of two protein isoforms that present different subcellular localizations, p32 (isoform 2) being mainly distributed throughout the cytoplasm, whereas p40 (isoform 1) is targeted to the nucleolus. MDFIC is known to down-regulate Tat-dependent transcription of HIV-1 LTR by interacting with HIV-1 Tat and Rev proteins and impairing their nuclear import (possibly by rendering the NLS domains inaccessible to importin-beta) and stimulate activation of human T-cell leukaemia virus type I (HTLV-I) LTR. In human cells, MDFIC binds to the axin complex, resulting in an increase in the level of free beta-catenin; it affects axin regulation of the WNT and JNK signalling pathways.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • HIC antibody
      • Human I mfa domain containing protein antibody
      • Human I-mfa domain-Containing protein antibody
      • I mfa domain containing protein antibody
      • MyoD family inhibitor domain containing protein antibody
      • MyoD family inhibitor domain-containing protein antibody
      • p32 antibody
      • p40 antibody
      see all

    Anti-MDFIC antibody images

    • Anti-MDFIC antibody (ab93224) at 1/100 dilution + HeLa cell lysate at 35 µg

      Predicted band size : 37 kDa

    References for Anti-MDFIC antibody (ab93224)

    ab93224 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab93224.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"