Overview

  • Product name
    Anti-MDH1 antibody [EPR13596(B)]
    See all MDH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13596(B)] to MDH1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MDH1 aa 50-150. The exact sequence is proprietary.
    Database link: P40925

  • Positive control
    • Human spleen, HepG2 and Jurkat lysates; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling MDH1 with ab181091 at 1/100 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • All lanes : Anti-MDH1 antibody [EPR13596(B)] (ab181091) at 1/2000 dilution

    Lane 1 : Human spleen lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

References

This product has been referenced in:
  • Kitazawa S  et al. Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors. Oncotarget 8:28922-28938 (2017). WB . Read more (PubMed: 28423651) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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