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Rabbit monoclonal [EPR13597(B)] to MDH1 - C-terminal
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MDH1 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P40925
Jurlat, HeLa and HepG2 cell lysates; HeLa cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Belongs to the LDH/MDH superfamily. MDH type 2 family.
Information by UniProt
Cytosolic malate dehydrogenase antibody
Diiodophenylpyruvate reductase antibody
Western blot - Anti-MDH1 [EPR13597(B)] antibody (ab180152)
All lanes : Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152) at 1/50000 dilution Lane 1 : Jurkat cell lysate Lane 2 : HeLa cell lysate Lane 3 : HepG2 cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution Predicted band size : 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MDH1 [EPR13597(B)] antibody (ab180152)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI.
Immunoprecipitation - Anti-MDH1 [EPR13597(B)] antibody (ab180152)
Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180152 at 1/50 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
Flow Cytometry - Anti-MDH1 [EPR13597(B)] antibody (ab180152)
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/20 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"