MDH2 Activity Assay Kit (ab119693)

Thank you very much!



The amount is not a problem...I can have as many flies as I want!



I think I would like to try the kit....BUT how can I buy the kit without knowing if I can use tecan reader?!

Any idea? My boss wont approve buying it....without knowing we have the machine to read it:) LOL



Bw

ANSWER:

Thank you for your reply. My colleague Cathrin is not in the office today.

Unfortunately, I do also not know if this kit is compatible with a Tecan reader.

I can suggest to ask the manufacturer of the Tecan reader. They should be able to help you after they had a look at the datasheet of protocol of this kit.

I am sorry I could not be of more help and hope this information is nevertheless helpful.

Do theywork on mice andDrosophilla? How specific is it? Tecan machine compatable (Flourescence)?Does the last buffer in the protocolincludes or need a detergent?

ANSWER:

Thank you for contacting us.

I have some good and some bad news for you. the good ones are that it will work in mice, and that the kits may work with Drosophila samples as we as the homology is for both enzymes compared with the human proteinsis over 98%. The bad one is that you will need quite an amount of material to get your experience running, and this may simply not possible with this test species.

These assays are specific to these enzymes and isoforms as demonstrated by mass spec analysis of immunoprecipitates.


Regarding the buffers, adetergent is not included in either final buffer.

And last but not least, unfortunately the lab couldn't tell me if your "Tecan reader" will work with the kits: they don't know it.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

uses human skeletal muscle biopsies from children (i.e. has limited samples)
How much tissue do I need to startwith?
Is this different for each kit?

ANSWER:

Thank you for contacting us.

I have heard back from the lab with the following information:

All these assays require different sample concentrations described in each booklet protocol - but in most cases is approximately 10ug/microplate well.
The good news is that normal whole muscle homogenate contains a good robust mitochondria signal so less is required, muscle samples are described in most if not all protocols.
Another good news is that the same preparation for any of the assays is suitable for all others. I would estimate that if a 1 mg of homogenate can be prepared (0.2mL at 5mg/mL).
This would provide enough material for each of the assays when diluted to the specific concentration in each assay.
We would recommend - if contemplating doing multiple assays - to include a mitochondrial sample as positive control such as ab110338 isolated mitochondria (note this is cow source)



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.