Overview

  • Product nameAnti-MDH2 antibody [2F5AF8]
    See all MDH2 primary antibodies
  • Description
    Mouse monoclonal [2F5AF8] to MDH2
  • Tested applicationsSuitable for: ICC/IF, In-Cell ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Tissue, cells or virus corresponding to Pig MDH2. Purified protein from porcine heart.

  • Positive control
    • Fibroblast cells Human heart homogenate Rat liver homogenate Mouse liver homogenate HepG2 whole cell lysate
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml.
IP Use at an assay dependent dilution.

Target

  • Sequence similaritiesBelongs to the LDH/MDH superfamily. MDH type 1 family.
  • Post-translational
    modifications
    Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • M MDH antibody
    • Malate dehydrogenase 2, NAD (mitochondrial) antibody
    • Malate dehydrogenase antibody
    • Malate dehydrogenase, mitochondrial antibody
    • MDH antibody
    • mdh2 antibody
    • MDHM_HUMAN antibody
    • MGC:3559 antibody
    • mitochondrial antibody
    • Mitochondrial malate dehydrogenase 2, NAD antibody
    • Mor 1 antibody
    • MOR1 antibody
    see all

Anti-MDH2 antibody [2F5AF8] images

  • Immunocytochemistry image of stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the ab110317 (1 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
  • ab110317 pulls down the 33kDa MDH2 protein from Human heart homogenate (1), Rat liver homogenate (2), Mouse liver homogenate (3) and HepG2 whole cell lysate (4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

References for Anti-MDH2 antibody [2F5AF8] (ab110317)

ab110317 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab110317.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"