The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Sequence similaritiesBelongs to the LDH/MDH superfamily. MDH type 1 family.
Post-translational modificationsAcetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%.