Anti-MDH2 antibody (ab96193)
Key features and details
- Rabbit polyclonal to MDH2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MDH2 antibody
See all MDH2 primary antibodies -
Description
Rabbit polyclonal to MDH2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Zebrafish -
Immunogen
Recombinant fragment corresponding to Human MDH2 aa 40-289.
Database link: P40926 -
Positive control
- 293T , A431 , HeLa , HepG2 , mouse brain and heart, rat lung.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
EDTA based, pH 8.0 buffer, 15min |
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ICC/IF |
1/100 - 1/1000.
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. EDTA based, pH 8.0 buffer, 15min |
ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
Target
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Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 1 family. -
Post-translational
modificationsAcetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 4191 Human
- Entrez Gene: 17448 Mouse
- Entrez Gene: 81829 Rat
- Omim: 154100 Human
- SwissProt: P40926 Human
- SwissProt: P08249 Mouse
- SwissProt: P04636 Rat
- Unigene: 520967 Human
see all -
Alternative names
- M MDH antibody
- Malate dehydrogenase 2, NAD (mitochondrial) antibody
- Malate dehydrogenase antibody
see all
Images
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All lanes :
Lane 1 : Wild-type 293T cell extract
Lane 2 : Knockout 293T cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE
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Immunofluorescence staining of ice-cold methanol-fixed (5 minutes) HeLa cells stained for MDH2 with ab96193 at 1:500 dilution. Blue: Hoechst 33342 staining.
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Immunohistochemical analysis of paraffin embedded mouse heart tissue labelling MDH2 with ab96193 antibody at 1/500 dilution at the mitochondria.
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All lanes : Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa -
Immunohistochemical analysis of paraffin embedded H1299 xenograft tissue labelling MDH2 with ab96193 antibody at 1/500 dilution.
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Mouse brain at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS PAGE
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Rat lung at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE
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All lanes : Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1 : Mouse C2C12 whole cell lysate
Lane 2 : Mouse C2C12 whole cell lysate on day 1 of differentiation
Lane 3 : Mouse C2C12 whole cell lysate on day 2 of differentiation
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab96193 has been referenced in 7 publications.
- Yuan M et al. Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging. Aging Dis 13:1532-1545 (2022). PubMed: 36186135
- Brinkley G et al. Teleological role of L-2-hydroxyglutarate dehydrogenase in the kidney. Dis Model Mech 13:N/A (2020). PubMed: 32928875
- Wongkittichote P et al. Tricarboxylic acid cycle enzyme activities in a mouse model of methylmalonic aciduria. Mol Genet Metab 128:444-451 (2019). PubMed: 31648943
- Shelar S et al. Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer. Clin Cancer Res 24:6433-6446 (2018). PubMed: 30108105
- Intlekofer AM et al. L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH. Nat Chem Biol 13:494-500 (2017). PubMed: 28263965
- Simsek D et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell 169:1051-1065.e18 (2017). PubMed: 28575669
- Naseri NN et al. Novel Metabolic Abnormalities in the Tricarboxylic Acid Cycle in Peripheral Cells From Huntington's Disease Patients. PLoS One 11:e0160384 (2016). WB . PubMed: 27611087