Overview

  • Product nameAnti-MDH2 antibody
    See all MDH2 primary antibodies
  • Description
    Rabbit polyclonal to MDH2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 40-289 of Human MDH2 (NP_005909).

  • Positive control
    • 293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab96193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.

Target

  • Sequence similaritiesBelongs to the LDH/MDH superfamily. MDH type 1 family.
  • Post-translational
    modifications
    Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • M MDH antibody
    • Malate dehydrogenase 2, NAD (mitochondrial) antibody
    • Malate dehydrogenase antibody
    • Malate dehydrogenase, mitochondrial antibody
    • MDH antibody
    • mdh2 antibody
    • MDHM_HUMAN antibody
    • MGC:3559 antibody
    • mitochondrial antibody
    • Mitochondrial malate dehydrogenase 2, NAD antibody
    • Mor 1 antibody
    • MOR1 antibody
    see all

Anti-MDH2 antibody images

  • Anti-MDH2 antibody (ab96193) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 36 kDa
  • All lanes : Anti-MDH2 antibody (ab96193) at 1/1000 dilution

    Lane 1 : Mouse C2C12 whole cell lysate
    Lane 2 : Mouse C2C12 whole cell lysate on day 1 of differentiation
    Lane 3 : Mouse C2C12 whole cell lysate on day 2 of differentiation

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit IgG at 1/100000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Anonymous Abreview.

    See Abreview

References for Anti-MDH2 antibody (ab96193)

ab96193 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Loading amount 20 µg
Specification C2C12
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Submitted Feb 26 2011

Application Western blot
Sample Rat Cell lysate - other (L6 myotubes)
Loading amount 20 µg
Specification L6 myotubes
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"