The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the LDH/MDH superfamily. MDH type 1 family.
Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%.
Immunohistochemical analysis of Human infiltrating duct carcinoma of breast tissue, staining MDH2 with ab181873 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.