Recombinant
RabMAb

Anti-MDH2 antibody [EPR14882(B)] (ab181873)

Overview

  • Product name
    Anti-MDH2 antibody [EPR14882(B)]
    See all MDH2 primary antibodies
  • Description
    Rabbit monoclonal [EPR14882(B)] to MDH2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MDH2 aa 100-200. The exact sequence is proprietary.
    Database link: P40926

  • Positive control
    • Jurkat, K562, HepG2 and HeLa cell lysates. Human infiltrating duct carcinoma of breast and mouse testis tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the LDH/MDH superfamily. MDH type 1 family.
  • Post-translational
    modifications
    Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • M MDH antibody
    • Malate dehydrogenase 2, NAD (mitochondrial) antibody
    • Malate dehydrogenase antibody
    • Malate dehydrogenase, mitochondrial antibody
    • MDH antibody
    • mdh2 antibody
    • MDHM_HUMAN antibody
    • MGC:3559 antibody
    • mitochondrial antibody
    • Mitochondrial malate dehydrogenase 2, NAD antibody
    • Mor 1 antibody
    • MOR1 antibody
    see all

Images

  • All lanes : Anti-MDH2 antibody [EPR14882(B)] (ab181873) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 36 kDa
  • Immunohistochemical analysis of Human infiltrating duct carcinoma of breast tissue, staining MDH2 with ab181873 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

  • Immunohistochemical analysis of mouse testis tissue, staining MDH2 with ab181873 at 1/50 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

References

This product has been referenced in:
  • Miikkulainen P  et al. HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma. Cancer Metab 5:5 (2017). Read more (PubMed: 28680592) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (15% acrylamide)
Sample
Pig Tissue lysate - other (Muscle Sarcoplasmic Extract)
Specification
Muscle Sarcoplasmic Extract
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Shannon Cruzen

Verified customer

Submitted Feb 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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