• Product name
  • Description
    Rabbit polyclonal to MDMX
  • Specificity
    Two bands were detected with the antibody in HeLa nuclear extract, at ~80 and 100 kDa. The 80 kDa band was no longer detected after the addition of competing peptide, suggesting that this band corresponds to MDMX. MDMX has been shown previously to migrate aberrantly at an ~80 kDa size (see deGraaf, et.al., J Biol Chem. 2003 Oct 3;278(40):38315-24). The 100 kDa band is not competed away by the addition of the immunizing peptide, suggesting that it is nonspecific cross-reactivity.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MDMX

    (Peptide available as ab16383.)

  • Positive control
    • This antibody gave a positive signal in the following nuclear cell extract: HeLa. This antibody gave a positive signal in the following Methanol fixed cell lines: HeLa.



Our Abpromise guarantee covers the use of ab16058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: Use at a concentration of 5 µg/ml.
    WB: 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 58 kDa). Can be blocked with MDMX peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
    • Tissue specificity
      Expressed in all tissues tested with high levels in thymus.
    • Sequence similarities
      Belongs to the MDM2/MDM4 family.
      Contains 1 RanBP2-type zinc finger.
      Contains 1 RING-type zinc finger.
      Contains 1 SWIB domain.
    • Domain
      Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
    • Post-translational
      Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp781B1423 antibody
      • Double minute 4 antibody
      • Double minute 4 human homolog of p53 binding protein antibody
      • Double minute 4 protein antibody
      • HDMX antibody
      • MDM 4 antibody
      • Mdm2 like p53 binding protein antibody
      • Mdm2-like p53-binding protein antibody
      • MDM4 antibody
      • Mdm4 p53 binding protein homolog mouse antibody
      • Mdm4 protein antibody
      • MDM4 related protein 1 antibody
      • Mdm4 transformed 3T3 cell double minute 4 antibody
      • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
      • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
      • MDM4_HUMAN antibody
      • Mdmx protein antibody
      • MGC132766 antibody
      • Mouse double minute 4 homolog antibody
      • Mouse double minute 4 human homolog of p53 binding protein antibody
      • MRP 1 antibody
      • MRP1 antibody
      • p53 binding protein antibody
      • p53 BINDING PROTEIN MDM4 antibody
      • p53-binding protein Mdm4 antibody
      • Protein Mdm4 antibody
      • Protein Mdmx antibody
      see all


    • All lanes : Anti-MDMX antibody (ab16058) at 1/1000 dilution

      Lane 1 : 20ug calf thymus histone
      Lane 2 : 20ug HeLa nuclear extract
      Lane 3 : 20ug calf thymus histone with Human MDMX peptide (ab16383) at 1 µg/ml
      Lane 4 : 20ug HeLa nuclear extract with Human MDMX peptide (ab16383) at 1 µg/ml

      Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 58 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa (possible cross reactivity).
    • ICC/IF image of ab16058 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16058, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).


    This product has been referenced in:
    • Xu XL  et al. CPUY201112, a novel synthetic small-molecule compound and inhibitor of heat shock protein Hsp90, induces p53-mediated apoptosis in MCF-7 cells. Sci Rep 6:19004 (2016). Read more (PubMed: 26743233) »
    • Zhuang C  et al. A novel drug discovery strategy: mechanistic investigation of an enantiomeric antitumor agent targeting dual p53 and NF-?B pathways. Oncotarget 5:10830-9 (2014). WB . Read more (PubMed: 25350970) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Thank you for your inquiry. The actual size was smaller than the predicted one, the reason can be thought that the post translational modification, e.g. phosphorylation, glycosylation etc. They increase the size of the protein. The 80 kDa band of ab1...

    Read More
    Immunocytochemistry/ Immunofluorescence
    Mouse Cell (fibroblast)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2%

    Dr. Jesus Espada

    Verified customer

    Submitted Jul 13 2006


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