Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Expressed in all tissues tested with high levels in thymus.
Belongs to the MDM2/MDM4 family. Contains 1 RanBP2-type zinc finger. Contains 1 RING-type zinc finger. Contains 1 SWIB domain.
Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
Immunoprecipitation of Jurkat cell lysates (1 mg for IP, 20% of IP loaded) using ab190364 at 6 µg/mg lysate (Lanes 1,2) or control IgG (Lane 3). Protein was detected using ab190364 at 1 µg/ml for western blotting.
Western blot - Anti-MDMX antibody (ab190364)
All lanes : Anti-MDMX antibody (ab190364) at 1 µg/ml
Lane 1 : Jurkat cell lysate at 50 µg Lane 2 : Jurkat cell lysate at 15 µg Lane 3 : Jurkat cell lysate at 5 µg