Overview

  • Product nameAnti-MDMX antibody [MDMX-82]
    See all MDMX primary antibodies
  • Description
    Mouse monoclonal [MDMX-82] to MDMX
  • Tested applicationsSuitable for: ELISA, IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Xenopus laevis, Chimpanzee, Opossum
  • Immunogen

    Synthetic peptide:

    KDPSPLYDMLRKNL

    , corresponding to amino acids 93-106 of Human MDMX.

Properties

Applications

Our Abpromise guarantee covers the use of ab49993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.

Target

  • FunctionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • Tissue specificityExpressed in all tissues tested with high levels in thymus.
  • Sequence similaritiesBelongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • DomainRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • Post-translational
    modifications
    Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Anti-MDMX antibody [MDMX-82] images

  • Anti-MDMX antibody [MDMX-82] (ab49993) at 1/1000 dilution + Mouse myoblast cell line C2C12 whole cell lysate at 15 µg

    Secondary
    HRP-conjugated sheep anti-mouse IgG polyclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-MDMX antibody [MDMX-82] (ab49993)

This product has been referenced in:
  • Endo S  et al. Potent in vitro and in vivo antitumor effects of MDM2 inhibitor nutlin-3 in gastric cancer cells. Cancer Sci 102:605-13 (2011). Human . Read more (PubMed: 21205074) »
  • Marine JC & Jochemsen AG Mdmx as an essential regulator of p53 activity. Biochem Biophys Res Commun 331:750-60 (2005). Read more (PubMed: 15865931) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (mouse myoblast cell line C2C12)
Specification mouse myoblast cell line C2C12
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Jul 25 2014

Thank you for your inquiry. The actual size was smaller than the predicted one, the reason can be thought that the post translational modification, e.g. phosphorylation, glycosylation etc. They increase the size of the protein. The 80 kDa band of ab1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"