Overview

  • Product name
    Anti-MDMX antibody [MDMX-82]
    See all MDMX primary antibodies
  • Description
    Mouse monoclonal [MDMX-82] to MDMX
  • Tested applications
    Suitable for: ELISA, IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Xenopus laevis, Chimpanzee, Opossum
  • Immunogen

    Synthetic peptide:

    KDPSPLYDMLRKNL

    , corresponding to amino acids 93-106 of Human MDMX.

Properties

Applications

Our Abpromise guarantee covers the use of ab49993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.

Target

  • Function
    Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • Tissue specificity
    Expressed in all tissues tested with high levels in thymus.
  • Sequence similarities
    Belongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • Domain
    Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • Post-translational
    modifications
    Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Images

  • Anti-MDMX antibody [MDMX-82] (ab49993) at 1/1000 dilution + Mouse myoblast cell line C2C12 whole cell lysate at 15 µg

    Secondary
    HRP-conjugated sheep anti-mouse IgG polyclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

    This image is courtesy of an anonymous Abreview

    See Abreview

References

This product has been referenced in:
  • Endo S  et al. Potent in vitro and in vivo antitumor effects of MDM2 inhibitor nutlin-3 in gastric cancer cells. Cancer Sci 102:605-13 (2011). WB ; Human . Read more (PubMed: 21205074) »
  • Marine JC & Jochemsen AG Mdmx as an essential regulator of p53 activity. Biochem Biophys Res Commun 331:750-60 (2005). Read more (PubMed: 15865931) »

See all 2 Publications for this product

Customer reviews and Q&As

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Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (mouse myoblast cell line C2C12)
Specification
mouse myoblast cell line C2C12
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jul 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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