Anti-MDMX (phospho S367) antibody [15] (ab122926)

Overview

  • Product nameAnti-MDMX (phospho S367) antibody [15]
    See all MDMX primary antibodies
  • Description
    Mouse monoclonal [15] to MDMX (phospho S367)
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence of Human MDXM protein surrounding phospho-Ser367.

  • Positive control
    • MCF cells reincubated with the proteasome inhibitor MG132 (20 uM) and exposed to DNA damaging agent, adriamycin (3 uM) or etoposide (20 uM).

Properties

Applications

Our Abpromise guarantee covers the use of ab122926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • FunctionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • Tissue specificityExpressed in all tissues tested with high levels in thymus.
  • Sequence similaritiesBelongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • DomainRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • Post-translational
    modifications
    Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Anti-MDMX (phospho S367) antibody [15] images

  • All lanes : Anti-MDMX (phospho S367) antibody [15] (ab122926) at 1 µg/ml

    Lane 1 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM)
    Lane 2 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 1 hour
    Lane 3 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 2 hour
    Lane 4 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 3 hour
    Lane 5 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 4 hour
    Lane 6 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 1 hour
    Lane 7 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 2 hour
    Lane 8 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 3 hour
    Lane 9 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 4 hour


    Predicted band size : 55 kDa

References for Anti-MDMX (phospho S367) antibody [15] (ab122926)

This product has been referenced in:
  • Okamoto K  et al. DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation. Mol Cell Biol 25:9608-20 (2005). Read more (PubMed: 16227609) »

See 1 Publication for this product

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