Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926)
Key features and details
- Mouse monoclonal [15] to MDMX/MDM4 (phospho S367)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-MDMX/MDM4 (phospho S367) antibody [15]
See all MDMX/MDM4 primary antibodies -
Description
Mouse monoclonal [15] to MDMX/MDM4 (phospho S367) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF cells reincubated with the proteasome inhibitor MG132 (20 uM) and exposed to DNA damaging agent, adriamycin (3 uM) or etoposide (20 uM).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
15 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. |
Target
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Function
Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. -
Tissue specificity
Expressed in all tissues tested with high levels in thymus. -
Sequence similarities
Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain. -
Domain
Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2. -
Post-translational
modificationsUbiquitinated. Deubiquitinated by USP2; leading to stabilize it. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4194 Human
- Omim: 602704 Human
- SwissProt: O15151 Human
- Unigene: 497492 Human
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Alternative names
- DKFZp781B1423 antibody
- Double minute 4 antibody
- Double minute 4 human homolog of p53 binding protein antibody
see all
Images
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All lanes : Anti-MDMX/MDM4 (phospho S367) antibody [15] (ab122926) at 1 µg/ml
Lane 1 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM)
Lane 2 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 1 hour
Lane 3 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 2 hour
Lane 4 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 3 hour
Lane 5 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to adriamycin (3 uM) for 4 hour
Lane 6 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 1 hour
Lane 7 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 2 hour
Lane 8 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 3 hour
Lane 9 : MCF cells preincubated with the proteasome inhibitor MG132 (20 uM), exposed to etoposide (20 uM) for 4 hour
Predicted band size: 55 kDaThe upper lanes show incubation with a non-phosphos antibody to MDMX/MDM4. The lower lanes as indicated above.
The cell lysates were immunoprecipitated with another anti-MDMX/MDM4 antibody before western blotting.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab122926 has been referenced in 2 publications.
- Zhang W et al. PTPRO-mediated autophagy prevents hepatosteatosis and tumorigenesis. Oncotarget 6:9420-33 (2015). WB ; Mouse . PubMed: 25826083
- Okamoto K et al. DNA damage-induced phosphorylation of MdmX at serine 367 activates p53 by targeting MdmX for Mdm2-dependent degradation. Mol Cell Biol 25:9608-20 (2005). PubMed: 16227609