Overview

  • Product nameAnti-MDMX (phospho S403) antibody
    See all MDMX primary antibodies
  • Description
    Rabbit polyclonal to MDMX (phospho S403)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic phospho-peptide corresponding to the region around Serine 403 of Human MDMX (SwissProt O15151)

Properties

Applications

Our Abpromise guarantee covers the use of ab70236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Detects a band of approximately 80 kDa.

Target

  • FunctionInhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
  • Tissue specificityExpressed in all tissues tested with high levels in thymus.
  • Sequence similaritiesBelongs to the MDM2/MDM4 family.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIB domain.
  • DomainRegion I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
  • Post-translational
    modifications
    Ubiquitinated. Deubiquitinated by USP2; leading to stabilize it.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781B1423 antibody
    • Double minute 4 antibody
    • Double minute 4 human homolog of p53 binding protein antibody
    • Double minute 4 protein antibody
    • HDMX antibody
    • MDM 4 antibody
    • Mdm2 like p53 binding protein antibody
    • Mdm2-like p53-binding protein antibody
    • MDM4 antibody
    • Mdm4 p53 binding protein homolog mouse antibody
    • Mdm4 protein antibody
    • MDM4 related protein 1 antibody
    • Mdm4 transformed 3T3 cell double minute 4 antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein antibody
    • Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse antibody
    • MDM4_HUMAN antibody
    • Mdmx protein antibody
    • MGC132766 antibody
    • Mouse double minute 4 homolog antibody
    • Mouse double minute 4 human homolog of p53 binding protein antibody
    • MRP 1 antibody
    • MRP1 antibody
    • p53 binding protein antibody
    • p53 BINDING PROTEIN MDM4 antibody
    • p53-binding protein Mdm4 antibody
    • Protein Mdm4 antibody
    • Protein Mdmx antibody
    see all

Anti-MDMX (phospho S403) antibody images

  • All lanes : Anti-MDMX (phospho S403) antibody (ab70236) at 0.13 µg/ml

    Lane 1 : 293T whole cell lysate with HA tagged-MDMX S403A mutant and NCS (2hrs at 200 ng/ml)
    Lane 2 : 293T whole cell lysate with HA tagged-MDMX S403A mutant and NCS (0.5hrs at 200 ng/ml)
    Lane 3 : 293T whole cell lysate with HA tagged-MDMX S403A mutant
    Lane 4 : 293T whole cell lysate with HA tagged-MDMX (wild-type) and NCS (2hrs at 200 ng/ml)
    Lane 5 : 293T whole cell lysate with HA tagged-MDMX(wild-type) and NCS (0.5hrs at 200 ng/ml)
    Lane 6 : 293T whole cell lysate with HA tagged-MDMX (wild-type)
    Lane 7 : mock transfected 293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique

    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References for Anti-MDMX (phospho S403) antibody (ab70236)

ab70236 has not yet been referenced specifically in any publications.

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