Overview

  • Product nameAnti-MDP1 antibody
    See all MDP1 primary antibodies
  • Description
    Rabbit polyclonal to MDP1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MARLPKLAVF DLDYTLWPFW VDTHVDPPFH KSSDGTVRDR RGQDVRLYPE) of Human MDP1 (NP_612485)

  • Positive control
    • HT1080 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IP Use at an assay dependent concentration.

Target

Anti-MDP1 antibody images

  • Anti-MDP1 antibody (ab99033) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Predicted band size : 20 kDa

References for Anti-MDP1 antibody (ab99033)

ab99033 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HEK293 cells)
Specification HEK293 cells
Treatment Over expressed with MDP1 plasmid
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Apr 10 2015

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (HEK293 cells)
Specification HEK293 cells
Treatment over expressed with MDP1 plasmid
Total protein in input 100 µg
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Submitted Apr 10 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Staph. aureus
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Total protein in input 100 µg
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Submitted Sep 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Sep 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MCF7)
Total protein in input 100 µg
Immuno-precipitation step Other - sepharose beads
Specification MCF7
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Submitted May 16 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted May 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"