Overview

  • Product nameMDR Assay Kit (Fluorometric)
    See all MDR kits
  • Tests
    1 x 96 well plate
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    1h 0m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Multi drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP dependent drug efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug resistance associated protein (MRP1) are members of the ABC transporter family. Abcam's Fluorimetric MDR Assay Kits use a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporters, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Abcam's Fluorimetric MDR Assay Kits are ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.

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  • Notes

    Store ab112142 dessicated.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112142 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    MDR Assay Kit (Fluorometric) images

    • Effect of Cyclosporin A on the inhibition of P-gp pump in MCF7/ADR cells. The increased concentration of Cyclosporin A resulted in an increase in fluorescence signal caused by the inhibition of P-gp pump which enhanced the intracellular accumulation of MDR indicator dye. The EC50 = 2.4 µM (measured with the kit) is similar to the value reported in the literature.

    Protocols

    References for MDR Assay Kit (Fluorometric) (ab112142)

    This product has been referenced in:
    • Wang S  et al. Evodiamine synergizes with doxorubicin in the treatment of chemoresistant human breast cancer without inhibiting P-glycoprotein. PLoS One 9:e97512 (2014). Functional Studies . Read more (PubMed: 24830744) »

    See 1 Publication for this product

    Product Wall

    The correct assay method for the kit ab112142 is read from bottom (fluorescence intensity in live cells) using a microplate with opaque wall (black or white) but a transparent bottom. The fluorescence intensity will increase (inside of the live cells) ...

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    We do not have in-house data for non-adherent cells but customers have reported using the assay with that kind of sample successfully. We do not have their data though. As far as we know, getting good results with cells in suspension is no more difficu...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"