Anti-ME1 antibody (ab223761)
Key features and details
- Rabbit polyclonal to ME1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ME1 antibody
See all ME1 primary antibodies -
Description
Rabbit polyclonal to ME1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig -
Immunogen
Recombinant fragment corresponding to Human ME1 aa 250-450.
Database link: P48163 -
Positive control
- WB: Human plasma (IgG/HSA depleted); Human tonsil lysate, IHC-P: Human adrenal gland tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 64 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 64 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed in all tissues tested including liver, placenta and white adipose tissue. -
Sequence similarities
Belongs to the malic enzymes family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4199 Human
- Entrez Gene: 17436 Mouse
- Entrez Gene: 397538 Pig
- Entrez Gene: 24552 Rat
- Omim: 154250 Human
- SwissProt: P48163 Human
- SwissProt: P06801 Mouse
- SwissProt: Q29558 Pig
see all -
Alternative names
- Cytosolic malic enzyme 1 antibody
- HUMNDME antibody
- Malate dehydrogenase antibody
see all
Images
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Paraffin-embedded human adrenal gland tissue stained for ME1 using ab223761 at 1/50 dilution in immunohistochemical analysis.
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All lanes : Anti-ME1 antibody (ab223761) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 64 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223761 has not yet been referenced specifically in any publications.