Overview

  • Product nameAnti-ME1 antibody
    See all ME1 primary antibodies
  • Description
    Rabbit polyclonal to ME1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: QQLNIHGLLP PSFNSQEIQV LRVVKNFEHL NSDFDRYLLL MDLQDRNEKL , corresponding to N terminal amino acids 36-85 of Human ME1 (NP_002386).

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

Anti-ME1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling ME1 with ab84561 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Cytoplasmic staining shown. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - ME1. Right - Merge.


  • Predicted band size : 64 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling ME1 with ab84561 at 1.0µg/ml.


  • Predicted band size : 64 kDa
    Western blot analysis of 293T cell lysate labeling ME1 with ab84561 at 1.0µg/ml.
  • Anti-ME1 antibody (ab84561) at 1 µg/ml (5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 34 kDa,80 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

References for Anti-ME1 antibody (ab84561)

ab84561 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (mouse stomach lysate)
Loading amount 20 µg
Specification mouse stomach lysate
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Ms. Sandra Gonzalez

Verified customer

Submitted Sep 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"