Overview

  • Product nameAnti-ME1 antibody
    See all ME1 primary antibodies
  • Description
    Mouse monoclonal to ME1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human ME1 protein (AAH25246) with a proprietary tag.

  • Positive control
    • WB: HepG2 and ME1 transfected 293T cell lysates IHC-P: human tonsil tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 64 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 µg/ml.

Target

  • Tissue specificityExpressed in all tissues tested including liver, placenta and white adipose tissue.
  • Sequence similaritiesBelongs to the malic enzymes family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytosolic malic enzyme 1 antibody
    • HUMNDME antibody
    • Malate dehydrogenase antibody
    • Malic enzyme 1 antibody
    • Malic enzyme 1, NADP(+) dependent, cytosolic antibody
    • Malic enzyme 1, soluble antibody
    • Malic enzyme, cytoplasmic antibody
    • MAOX_HUMAN antibody
    • ME1 antibody
    • MES antibody
    • NADP dependent malic enzyme antibody
    • NADP ME antibody
    • NADP-dependent malic enzyme antibody
    • NADP-ME antibody
    • Pyruvic malic carboxylase antibody
    see all

Anti-ME1 antibody images

  • Anti-ME1 antibody (ab87974) at 5 µg/ml + recombinant human ME1 protein with a proprietary tag (the immunogen) at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 64 kDa
    Observed band size : 89 kDa (why is the actual band size different from the predicted?)
  • Anti-ME1 antibody (ab87974) at 5 µg/ml + HepG2 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 64 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ME1 antibody (ab87974) at 5 µg/ml

    Lane 1 : ME1 transfected lysate from 293T cell line
    Lane 2 : Non transfected lysate from 293T cell line

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • ab87974, at 0.5 µg/ml, staining ME1 in formalin fixed, paraffin embedded human tonsil tissue sections by Immunoperoxidase/Immunohistochemistry.
  • ab87974, detecting recombinant tagged ME1 by sandwich ELISA. The detection limit is approximately 1 ng/ml as a capture antibody.

References for Anti-ME1 antibody (ab87974)

ab87974 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"