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Rabbit polyclonal to ME1
Mouse, Rat, Human
Predicted to work with:
Recombinant fragment corresponding to a region within amino acids 161-489 of Human ME1 (NP_002386).
WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates
ICC/IF: HeLa cell
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 64 kDa.
1/100 - 1/200.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Expressed in all tissues tested including liver, placenta and white adipose tissue.
Belongs to the malic enzymes family.
Information by UniProt
Cytosolic malic enzyme 1 antibody
Malate dehydrogenase antibody
Western blot - Anti-ME1 antibody (ab97445)
All lanes : Anti-ME1 antibody (ab97445) at 1/500 dilution Lane 1 : A431 whole cell lysate Lane 2 : HeLa S3 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 64 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-ME1 antibody (ab97445)
Immunofluorescence analysis of ME1 in paraformaldehyde fixed HeLa, using ab97445 at a 1/200 dilution.
Lower image: merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME1 antibody (ab97445)
IHC image of ab97445 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond
TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"