IHC image of ab97445 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-ME1 antibody (ab97445)
This product has been referenced in:
Al-Dwairi A et al. Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice. PLoS One9:e113058 (2014).
Read more (PubMed: 25402228) »
Fritz V et al. Metabolic intervention on lipid synthesis converging pathways abrogates prostate cancer growth. Oncogene32:5101-10 (2013).
Read more (PubMed: 23208508) »