Overview

  • Product nameAnti-ME1 antibody
    See all ME1 primary antibodies
  • Description
    Rabbit polyclonal to ME1
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 161-489 of Human ME1 (NP_002386).

  • Positive control
    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab97445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
ICC/IF 1/100 - 1/200.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Anti-ME1 antibody images

  • All lanes : Anti-ME1 antibody (ab97445) at 1/500 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 64 kDa
  • Immunofluorescence analysis of ME1 in paraformaldehyde fixed HeLa, using ab97445 at a 1/200 dilution.
    Lower image: merged with DNA probe.
  • IHC image of ab97445 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ME1 antibody (ab97445)

This product has been referenced in:
  • Al-Dwairi A  et al. Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice. PLoS One 9:e113058 (2014). WB ; Mouse . Read more (PubMed: 25402228) »
  • Fritz V  et al. Metabolic intervention on lipid synthesis converging pathways abrogates prostate cancer growth. Oncogene 32:5101-10 (2013). WB ; Mouse . Read more (PubMed: 23208508) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Ileum)
Loading amount 100 µg
Specification Ileum
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Ahmed Nayef

Verified customer

Submitted Oct 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"