Anti-ME1 antibody (ab97445)
Key features and details
- Rabbit polyclonal to ME1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-ME1 antibody
See all ME1 primary antibodies -
Description
Rabbit polyclonal to ME1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 161-489 of Human ME1 (NP_002386).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 64 kDa.
|
ICC/IF |
1/100 - 1/200.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 64 kDa. |
ICC/IF
1/100 - 1/200. |
Target
-
Tissue specificity
Expressed in all tissues tested including liver, placenta and white adipose tissue. -
Sequence similarities
Belongs to the malic enzymes family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 4199 Human
- Entrez Gene: 17436 Mouse
- Entrez Gene: 24552 Rat
- Omim: 154250 Human
- SwissProt: P48163 Human
- SwissProt: P06801 Mouse
- SwissProt: P13697 Rat
- Unigene: 21160 Human
see all -
Alternative names
- Cytosolic malic enzyme 1 antibody
- HUMNDME antibody
- Malate dehydrogenase antibody
see all
Images
-
All lanes : Anti-ME1 antibody (ab97445) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 64 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of ME1 in paraformaldehyde fixed HeLa, using ab97445 at a 1/200 dilution.
Lower image: merged with DNA probe.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (21)
ab97445 has been referenced in 21 publications.
- Jiang Z et al. miR-885-5p Inhibits Invasion and Metastasis in Gastric Cancer by Targeting Malic Enzyme 1. DNA Cell Biol 40:694-705 (2021). PubMed: 33751897
- Wei X et al. Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats. Cell Rep 32:108207 (2020). PubMed: 32997989
- Li H et al. CMap analysis identifies Atractyloside as a potential drug candidate for type 2 diabetes based on integration of metabolomics and transcriptomics. J Cell Mol Med 24:7417-7426 (2020). PubMed: 32469143
- Shao C et al. Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis. Redox Biol 36:101685 (2020). PubMed: 32853879
- Ghergurovich JM et al. A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway. Nat Chem Biol 16:731-739 (2020). PubMed: 32393898