Overview

  • Product name
    Anti-ME2 antibody [EP7217]
    See all ME2 primary antibodies
  • Description
    Rabbit monoclonal [EP7217] to ME2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ME2 aa 100-200. The exact sequence is proprietary.

  • Positive control
    • Jurkat, K562, HL60, 293T and HeLa cell lysates; Human kidney tissue and papillary adenocarcinoma of thyroid tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab139686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Overlay histogram showing HeLa cells stained with ab139686 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139686, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-ME2 antibody [EP7217] (ab139686) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 65 kDa

  • Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma of thyroid tissue labeling ME2 with ab139686 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ME2 with ab139686 at 1/50 dilution.
  • Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab139686 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab139686 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab139686 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab139686 showing +ve staining.

References

This product has been referenced in:
  • Dey P  et al. Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature 542:119-123 (2017). WB ; Human . Read more (PubMed: 28099419) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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